Occurrence dataset Registered July 30, 2021
The InBIO Barcoding Initiative Database: Hemiptera 01
Description
The dataset contains 135 records of Hemiptera species collected from 2015 to 2019 in continental Portugal (Sousa et al., 2021). The species represented in the dataset, 90 in total, correspond to about 7.5% of the known true bugs diversity of continental Portugal, and contribute to the knowledge on the DNA barcodes and distribution of Portuguese Hemiptera. Specimens were captured during fieldwork by direct search of specimens or by sweeping the vegetation and stored in 96% ethanol. All specimens were identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Purpose
The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
Geographic scope
- Description
Continental Portugal.
- Latitude
- From 37.257 to 41.979
- Longitude
- From -9.465 to -6.344
Temporal scope
- range
- March 16, 2015 - September 20, 2019
Taxonomic scope
- Description
This dataset is composed of data relating to 135 Hemiptera specimens. All specimens were determined to species level or below. Overall, 90 species and one subspecies are represented in the dataset. These species belong to 31 families. The Pentatomidae family accounts for 20% of the total collected specimens, and no other family accounts for more than 8%. The Pentatomidae and Miridae families combined account for 26% of the total taxa represented, and no other family accounts for more than 7%. Eleven families are represented by a single taxon and ten by two taxa.
- Coverage
- Animalia
Arthropoda
Insecta
Hemiptera
Acanthosomatidae
Methodology
- Sampling
The studied material was collected in 60 different localities from continental Portugal. Sampling was conducted between 2015 and 2019 on a wide range of habitats, both by direct search of specimens or using a hand-held sweep net. Collected specimens were examined using a binocular stereoscopic microscope and stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed based on keys and descriptions from literature.
- Study extent
Specimens were collected in 60 different localities of continental Portugal. Sampling was conducted from 2015 to 2019, in several field work expeditions.
- Quality control
All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org).
- Method steps
Specimens were collected in 60 different localities of continental Portugal. Fieldwork was carried out between 2015 and 2019.
Specimens were collected during fieldwork by direct search of specimens or by sweeping the vegetation and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
Specimens were morphologically identified with the assistance of a stereoscopic microscope and using the available literature. A subset (23%) was identified using the BOLD Identification Engine directly.
DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. For one specimens of Ceraleptus lividus only a 418 bp fragment was sequenced. DNA extracts were deposited in the IBI collection.
All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen, when available, was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
Metrics
Bibliography
- Google ScholarSousa, P., Grosso-Silva, J.M., Andrade, R., Chaves, C., Pinto, J., Paupério, J., Beja, P. & Ferreira, S. (2021). The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese Hemiptera 01. Biodiversity Data Journal, 9: e65314. doi: 10.3897/BDJ.9.e65314
Contacts
- Organization
- CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
- Position
- Post-Doc Researcher
- Address
- Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
- Roles
- Originator
Metadata author - Phone
- User ID
- Organization
- Museu de História Natural e da Ciência da Universidade do Porto
- Position
- Entomology Curator
- Roles
- Originator
Metadata author - User ID
- Position
- Researcher
- Address
- Rua Calouste Gulbenkian, 237
- Roles
- Originator
Metadata author
- Organization
- CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
- Position
- Coordinating Researcher
- Address
- Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
- Roles
- Originator
Metadata author
Administrative point of contact - Phone
- User ID
- Organization
- CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
- Position
- Post-Doc Researcher
- Address
- Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
- Roles
- Originator
Metadata author
Administrative point of contact - Phone
- User ID
GBIF registration
- Registration date
- July 30, 2021
- Metadata last modified
- July 30, 2021
- Publication date
- July 30, 2021
- Hosted by
- Instituto Superior de Agronomia / Universidade de Lisboa
- Installation
- GBIF Portugal IPT
- Endpoints
- Darwin Core Archive
- EML
- Preferred identifier
- 10.15468/8hn25y
- Alternative identifiers